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The facility is located on 235 acres (0.95 km²) along the banks of the Brandywine River, the site of the gunpowder mill founded by Eleuthère Irénée du Pont in 1802. Today, the facility offers a diversity of restorations, exhibits, and live demonstrations for visitors.
The museum and grounds include the first du Pont family home and garden in the United States, the powder yards, and a nineteenth-century machine shop.