Genetic Engineering Naming Applications Genetic Engineering And

It often involves the isolation, manipulation and reintroduction of DNA into model organisms, usually to express a protein. The aim is introduce new genetic characteristics to an organism to increase its usefulness such as, increasing the yield of a crop species, introducing a novel characteristic, or producing a new protein or enzyme. Examples are the production of human insulin through the use of modified bacteria and the production of new types of mice like the OncoMouse, (cancer mouse) for research, through genetic redesign.

Since a protein is specified by a DNA segment or gene, future copies of that protein can be modified by changing the gene's underlying DNA. One way to do this is to isolate the DNA, cut it, and splice in a different DNA segment. Daniel Nathans and Hamilton Smith received the 1978 Nobel Prize in physiology or medicine for their isolation of restriction endonucleases, which are able to cut DNA at specific sites. Together with ligase, which can join together fragments of DNA, restriction enzymes formed the initial basis of recombinant DNA technology.

1 Naming

Genetic modification or genetic manipulation are claimed to be neutral and possibly more technically correct terms for what is claimed, controversially, to be genetic engineering. Opponents question whether the concept of 'modification', with its implications of progress, are applicable here.

Many opponents of the use of the term 'genetic engineering' argue the operations of genes in combination with cell biochemistry are rather poorly understood and sometimes lead to unexpected side effects.

Reluctance to recognize this field as "engineering" has become popular in the anti-globalization movement and safe trade movement, and is also widely held by most Green parties, and the major parties of FranceThe French Republic or France ( French: Republique francaise or France is a country whose metropolitan territory is located in western Europe, and which is further made up of a collection of overseas islands and territories located in other continents. and GermanyThe Federal Republic of Germany ( German: Bundesrepublik Deutschland is one of the world's leading industrialized countries, located in the middle of the European Union. It is bordered to the north by the North Sea, Denmark and the Baltic Sea, to the east, which have resisted any agricultural policyAn agricultural policy or agricultural subsidy is an incentive to engage in a particular form of agriculture. It often takes the form of tax reductions, favorable deals on equipment, and so on. Subsidies status Currently, economic studies place the averag favoring genetically modified foodA genetically modified food is a food product containing some quantity of any genetically modified organism (GMO) as an ingredient. They are mainly to increase the mass of food to feed more people. Some nations have very strong disagreement over genetical. These groups tend to resist the label 'engineer' as applied to such genetic modification most strongly.

Defenders of the term genetic engineering argue that animal husbandryAnimal husbandry is the agricultural practice of breeding and raising livestock. As such, it is a vital skill for farmers, and in many ways as much art as it is science. The science of animal husbandry, called animal science is taught in many universities and crop breeding are also forms of genetic engineering that use artificial selectionChihuahua mix and Great Dane show the wide range of dog breed sizes created using artificial selection. In the theory of evolution, artificial selection is the process of intentional or unintentional modification of a species through human actions which e instead of modern genetic modification techniques. It is politics, they argue, not economics or science, that causes their work to be closely investigated, and for different standards to apply to it than to other fields of engineeringEngineering is the application of science to the needs of humanity. This is accomplished through knowledge, mathematics, and practical experience applied to the design of useful objects or processes. Professional practitioners of engineering are called en. These scientists, however, do not object to the term 'genetic modification' as applied to what they do, although it is sometimes used to deny them the status of professionals serving society in an ethical manner, which is one implication of the term engineer.

The term "genetic engineering" is sometimes informally abbreviated as "genegineering."

" Transgenic organism" is now the preferred term for genetically modified organisms with extra-genome information, as opposed to "genetically engineered" organisms.

2 Applications

One of the best known applications of genetic engineering is genetically modified organisms (GMOs).

There are potentially momentous biotechnology applications of GM, for example oral vaccines produced naturally in fruit at very low cost. This represents, however, a spread of genetic modification to medical purposes and opens an ethical door to other uses of the technology to directly modify human genomes.

These effects are often not traceable back to direct causes in the genome, but rather in the environment or interaction of proteins. The means by which 'genes' (in fact DNA strands that are assumed to have discrete effects) are detected and inserted are inexact, including such means as coating gold particles with DNA to be inserted and literally firing it at strands of target DNA (see gene gun), which is guaranteed to cause insertions in at least some random locations, which can on rare occasion cause unplanned characteristics.

Similar objections apply to protein engineering and molecular engineering for use as drugs. However, a single protein or a molecule is easier to examine for ' quality control' than a complete genome, and there are more limited claims made for the reliability of proteins and molecules, than for the genomes of whole organisms. While protein and molecule engineers often times acknowledge the requirement to test their products in a wide variety of environments to determine if they pose dangers to life, the position of many genetic engineers is that they do not need to do so, since the outputs of their work are 'substantially the same as' the original organism which was produced by the original genome(s).

An extreme ambition of some groups is human enhancement via genetics, eventually by artificial intelligence or molecular engineering. See also: transhumanism.

2.1 Genetic engineering and research

Although a there has been a tremendous revolution in the biological sciences in the past twenty years, there is still a great deal that remains to be discovered. The completion of the sequencing of the human genome, as well as the genomes of most agriculturally and scientifically important plants and animals, have increased the possibilities of genetic research immeasurably. Expedient and inexpensive access to comprehensive genetic data has become a reality, with billions of sequenced nucleotides already online and annotated. Now that the rapid sequencing of arbitrarily large genomes has become a simple, if not trivial affair, a much greater challenge will be elucidating function of the extraordinarily complex web of interacting proteins, dubbed the proteome, that constitutes and powers all living things. Genetic engineering has become the gold standard in protein research, and major research process has been made using a wide variety of techniques, including

loss of function, such as in a knockout experiment, in which an organism is engineered to lack one or more genes. This allows the experimenter to analyze the defects caused by this mutation, and can be considerably useful in unearthing the function of a gene. It is used especially frequently in developmental biology. A knockout experiment involves the creation and manipulation of a DNA construct in vitro, which, in a simple knockout, consists of a copy of the desired gene which has been slightly altered such as to cripple its function. The construct is then taken up by embryonic stem cells, where the engineered copy of the gene replaces the organism's own gene. These stem cells are injected into blastocysts, which are implanted into surrogate mothers. Another method, useful in organisms such as drosophila, is to induce mutations in a large population and then screen the progeny for the desired mutation. A similar process can be used in both plants and prokaryotes.
gain of function experiments, the logical counterpart of knockouts. These are sometimes performed in conjunction with knockout experiments to more finely establish the function of the desired gene. The process is much the same as that in knockout engineering, except that the construct is designed to increase the function of the gene, usually by providing extra copies of the gene or attracting more frequent transcription.
'tracking' experiments, which seek to gain information about the localization and interaction of the desired protein. One way to do this is to replace the wild-type gene with a 'fusion' gene, which is a juxtaposition of the wild-type gene with a reporting element such as green fluorescent protein that will allow easy visualization of the products of the genetic modification. While this is a useful technique, the manipulation can destroy the function of the gene, creating secondary effects and possibly calling into question the results of the experiment. More sophisticated techniques are now in development that can track protein products without mitigating their function, such as the addition of small sequences which will serve as binding motifs to monoclonal antibodies.

3 Ethics

Proponents of genetic engineering argue that the technology is not harmful, and that it is necessary in order to maintain food production that will continue to match population growth. However, others argue that food distribution, not production, is the problem, citing that the population growth is actually a result of uneven distribution of food (and wealth).

Others oppose genetic engineering on the grounds that genetic modifications may have unforeseen consequences, both in the initially modified organisms, and their environments. For example, certain strains of maize have been developed that are toxic to plant eating insects (see bt corn). However, when those strains cross-polinated with other varieties of wild and domestic maize, the relevant genes were passed on in unintended ways. This introduced a new gene into the gene pool of the maize population outside of the crop field.

Anti-genetic-engineering groups propose that genetic releases such as this represent the opening of a Pandora's box which may ultimately accelerate the collapse of the modern system of agriculture, decreasing rather than increasing the food supply. They say that with current recombinant technology there is no way to ensure that genetically modified organisms remain under control, and the use of this technology outside of secure laboratory environments carries grave risks for the future.

Many also fear that certain types of genetically engineered crops will enable the elimination of all biodiversity in the cropland; herbicide-tolerant crops will for example be treated with the relevant herbicide to the extent that there are no wild plants ('weeds') able to survive, and plants toxic to insects will mean insect-free crops. This could result in major declines in other wildlife (e.g. birds) which depend on weed seeds and/or insects for food resources. The recent (2003) farm scale studies in the UK found this to be the case with GM sugar beet and GM rapeseed, but not with GM maize (though in the last instance, the non-GM comparison maize crop had also been treated with environmentally damaging pesticides subsequently (2004) withdrawn from use in the EU).

Proponents of current genetic techniques as applied to food plants cite the benefits that the technology can have, for example, in the harsh agricultural conditions of third world countries. They say that with modifications, existing crops would be able to thrive under the relatively hostile conditions providing much needed food to their people. While submitting that precautions should be made to ensure that any modified crops are contained, they say that their genetically engineered crops are not significantly different from those modified by nature or humans in the past, and by extension are not dangerous to other crops. There is gene transfer between unicellular eukaryotes and prokaryotes. There have been no known genetic catastrophes as a result of this.

3.1 Economic and political effects

Many opponents of current genetic engineering believe the increasing use of GM in major crops has caused a power shift in agriculture towards Biotechnology companies gaining far greater control over the production chain of crops and food then any previous industry, and over the farmers that use their products, as well.

Many proponents of current genetic engineering techniques believe it will bring higher yields and profitability to many farmers, especially those in third world countries.

In April 2004 Hugo Chávez announced a total ban on genetically modified seeds in Venezuela.

4 See also:

5 External links

Ethics Molecular genetics

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