Home > Fluorescence spectroscopy

Contents

1 Fluorometer

1.1 Types

2 Analysis of data

3 Applications

4 See also

Fluorescent spectroscopy or fluorometry is a type of electromagnetic spectroscopy used for analyzing fluorescent spectra. It involves using a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit light of a lower energy, typically, but not necessarily, visible light.

1 Fluorometer

A fluorometer measures fluorescence.

1.1 Types

Filter fluorometers use filters to isolate the incident light and fluorescent light.
Spectrofluorometer s use diffraction grating monochromators to isolate the incident light and fluorescent light. In these devices, the spectrum can consist of the intensity of emitted light as a function of the wavelength of either the incident light (excitation spectrum) or the emitted light, or both.

2 Analysis of data

There is a relationship between concentration and fluorescence intensity.

3 Applications

It used in, among others, biochemical, medical, and chemical research fields, for analysing organic compounds. There has been report of its use in differentiating malign skin tumours from benign.

4 See also

X-ray fluorescence spectroscopy .
Beer's law

SpectroscopySpectroscopy is the study of spectra, ie. the dependence of a physical measure to frequency. Spectroscopy is often used in physical and analytical chemistry for the identification of substances, through the spectrum emitted or absorbed. A device for recor

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Topics: Fluorescence Spectroscopy, Fluorometer, Types, Analysis Of Data...

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Fluorescence microscope

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